cDNA microarray analysis using both gene expression ratios and intensities
نویسندگان
چکیده
منابع مشابه
Assessment of gene expression profile by cDNA microarray analysis.
With the advent of cDNA microarray technology, variations in cellular transcriptional events can now be monitored simultaneously, and expression profile at each functional state can be established and compared to one another. Here we illustrate gene expression profile analysis of a cisplatin-resistant ovarian carcinoma cell line (A2780CP)and its parental A2780 cell line, and compare them to ass...
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DNA microarray technology is a new and powerful tool for studying a genome-wide gene expression in many organisms. This technology is a high throughput, highly parallel RNA expression assay technique that permits quantitative analysis of RNAs transcribed from both known and unknown genes. This review describes array manufacturing, experimental design, and its application to gene discovery and e...
متن کاملWhich is better for cDNA-microarray-based classification: ratios or direct intensities
MOTIVATION There are two general methods for making gene-expression microarrays: one is to hybridize a single test set of labeled targets to the probe, and measure the background-subtracted intensity at each probe site; the other is to hybridize both a test and a reference set of differentially labeled targets to a single detector array, and measure the ratio of the background-subtracted intens...
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Polycythemia vera (PV) is a myeloproliferative disorder characterized by an increased proliferation of all three myeloid lineages. The molecular pathogenesis of PV is unknown. Using cDNA microarrays comprising 6000 human genes, we studied the gene expression profile of granulocytes obtained from 11 PV patients compared with granulocytes obtained from healthy individuals. We found that 147 genes...
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Polycythemia vera (PV) is a myeloproliferative disorder characterized by an increased proliferation of all three myeloid lineages. The molecular pathogenesis of PV is unknown. Using cDNA microarrays comprising 6000 human genes, we studied the gene expression profile of granulocytes obtained from 11 PV patients compared with granulocytes obtained from healthy individuals. We found that 147 genes...
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ژورنال
عنوان ژورنال: Nature Genetics
سال: 2001
ISSN: 1061-4036,1546-1718
DOI: 10.1038/87032